Journal: Neurobiology of aging

Article Title: Prostate stem cell antigen interacts with nicotinic acetylcholine receptors and is affected in Alzheimer's disease.

doi: 10.1016/j.neurobiolaging.2015.01.001

Figure Lengend Snippet: Fig. 1. Prostate stem cell antigen (PSCA) is soluble and co-purifies with the a4 nAChR subunit in human cortex. (A) The antiserum directed against PSCA recognizes the human recombinant GST-tagged PSCA protein (total molecular weight 34.2 kDa) from 1 to 16 ng/well at the expected band size. Endogenous PSCA from mouse and human cortical tissue (8 mg total protein/well) is detected at approximately 24 kDa, and the amount in the cortical samples was compared with the known concentrations of re- combinant PSCA protein. (B) Images of Western blot showing PSCA protein levels in cortical tissue from human (protein concentration 4, 8, and 12 mg/well) and mouse (8, 15, and 30 mg/well) in the absence (peptide) and the presence of PSCA peptide (þpeptide). (C) Representative images of Western blots showing PSCA and Lypd6 protein levels in soluble and membrane fractions of human temporal cortical tissue. The membrane receptor proteins b2 nAChR and GluR2 are used as control. (D) Mag- netic beads covalently coupled with PSCA recombinant protein were incubated with cortical homogenates from human temporal cortex followed by detection of nAChR subunits by Western blot. Homogenates before (input) and after affinity purification (output) as well as the negative control (CTRL) were loaded.

Article Snippet: Validation of PSCA antibody (1:1,000, Novus Biologicals) was done with co-incubation with the PSCA peptide, which had been used as immunogen (1:100, # NB100-91938PEP, Novus Biologicals).

Techniques: Recombinant, Molecular Weight, Western Blot, Protein Concentration, Membrane, Control, Incubation, Negative Control

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Association between PSCA SNP rs2294008 and risk of cervical cancer.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques:

Journal: Scientific Reports

Article Title: PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population

doi: 10.1038/srep23465

Figure Lengend Snippet: Upper panel, representative images were obtained at 400× magnification. The frequency distribution of the CC, CT, and TT genotypes of rs2294008 was 25, 19, and 6, respectively. Bottom, the histogram of the PSCA expression in each genotype. ** P < 0.01.

Article Snippet: Series of paraffin sections of specimens were incubated with polyclonal rabbit anti-PSCA antibody (BA1694, BOSTER, Wuhan, China) overnight at 4 °C, and 3,3′-diaminobenzidine (DAB; Zhongshan Biotech, Beijing, China) was used to produce a brown precipitate.

Techniques: Expressing

Journal: Biomedicines

Article Title: Differential Protein-Coding Gene Expression Profile in Patients with Prostate Cancer

doi: 10.3390/biomedicines12112509

Figure Lengend Snippet: Genes classified as drug targets in the DrugBank database. Protein-coding genes as targets for drugs related to the treatment of prostate cancer in all states (investigation, experimental, approved). In the case of target genes for many drugs, only the first 10 are reported.

Article Snippet: DB05933 , MK-4721 , A fully human monoclonal antibody directed to Agensys’ proprietary target Prostate Stem Cell Antigen (PSCA), an antigen expressed at significant levels on tumor cells from the majority of patients with all stages of prostate, pancreatic, and bladder cancers [investigation]. , PSCA [O43653].

Techniques: Diagnostic Assay, Positron Emission Tomography, Membrane, Imaging, Biomarker Discovery, Expressing, Derivative Assay